RNA Purification
- Product Code: RNA Purification
mRNA Purification Kit from Total RNA (Biobasic)
This kit provides a convenient and easy-to-follow method for purification of mRNA from total RNA. Each prep can use up to 10ug total RNA as
starting material. mRNA is selectively bound on oligo-dT cellulose, while 99% of the remaining RNA, including rRNAs and tRNAs, do not bind.
Purified mRNA can be used for Norhtern blotting, RT-PCR, in vitro translation, differential display, cDNA synthesis or library construction, among others.
Features:
1. Fast. The entire procedure takes only 30 minutes.
2. High Quality of mRNA. OD260/OD280 of purified mRNA is generally >1.8.
3. Compatible with many downstream applications.
4. Easy to scale up.
| Cat no. | Supplier | Items | Quantity |
| GB0244-1KG | Bio Basic Inc. | Guanidine thiocyanate (Guanidine isothiocynate) CH5N3.CHNS MW 118.16, Purity > 99.5%, pH 4.5-6.0, DNase, RNase, Protease: non-detected | 1kg |
| GB0244-250G | Bio Basic Inc. | Guanidine thiocyanate (Guanidine isothiocynate) CH5N3.CHNS MW 118.16, Purity > 99.5%, pH 4.5-6.0, DNase, RNase, Protease: non-detected | 250g |
| GB0244-1KG | Bio Basic Inc. | Guanidine hydrochloride CH5N3.HCl MW 95.53, Purity >99.0%, Solubility 6M in water, pH 4.5-6.0, residue on ignition <0.05% Loss on drying <0.05% DNase, RNase, Protease: non-detected | 100g |
| GB0242-500G | Bio Basic Inc. | Guanidine hydrochloride CH5N3.HCl MW 95.53, Purity >99.0%, Solubility 6M in water, pH 4.5-6.0, residue on ignition <0.05% Loss on drying <0.05% DNase, RNase, Protease: non-detected | 500g |
| LDB0307 | Bio Basic Inc. | Lithium chloride, anhydrous LiCl MW 42.39, MP 605°C, BP 1382°C, Purity >99%, Sulfate<0.01%, Heavy metal < 10ppm | 250g |
| PD0252 | Bio Basic Inc. | Phenol (Carbolic acid) Double distilled for molecular biology C6H6O MW 94.11, Purity >99.5%, Density 1.053, MP 40-41°C, Water <0.5%, RNase, DNase: non-detected, Fe <1ppm, Mg < 1ppm, Heavy metals < 1ppm | 500g |
| PC6910-100ML | Bio Basic Inc. | Phenol, saturate (pH 7.9), Saturated with Tris-HCl | 100ml |
| PC6910-400ML | Bio Basic Inc. | Phenol, saturate (pH 7.9), Saturated with Tris-HCl | 400ml |